53 research outputs found

    Insight:an application of information visualisation techniques to digital forensics investigations

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    As digital devices are becoming ever more ubiquitous in our day to day lives, more of our personal information and behavioural patterns are recorded on these devices. The volume of data held on these devices is substantial, and people investigating these datasets are facing growing backlog as a result. This is worsened by the fact that many software tools used in this area are text based and do not lend themselves to rapid processing by humans.This body of work looks at several case studies in which these datasets were visualised in attempt to expedite processing by humans. A number of different 2D and 3D visualisation methods were trialled, and the results from these case studies fed into the design of a final tool which was tested with the assistance of a group of individuals studying Digital Forensics.The results of this research show some encouraging results which indicate visualisation may assist analysis in some aspects, and indicates useful paths for future work

    Ground plane rectification from crowd motion

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    This work focuses on the estimation of the ground-plane parameters needed to rectify and reconstruct crowded pedestrian scenes, projected into 2D by an uncalibrated, monocular camera. Deformities introduced during the imaging process affect metrics such as size, velocity and distance, which are often useful when examining the behaviour of agents within the scene. A framework is presented to reverse ā€œperspective distortionā€ by calculating the ā€œgroundplaneā€, upon which motion within the scene occurs. Existing methods use geometric features, such as parallel lines, or objects of known size, such as the height of individuals in the scene; however these features are often unavailable in densely crowded scenes due to occlusions. By measuring only the imaged velocity of tracked features, assumed to be constant in the world, the issue of occlusion can be largely overcome. A novel framework is presented for estimation of the ground-plane and camera focal-length for scenes modelled with a single plane. The above assumption is validated against simulations, outperforming an existing technique [12] against real-world benchmark data. This framework is extended into a two-plane world and the additional challenge of determining the respective topology of the planes is introduced. Several methods for locating the intersection-line between the two planes are evaluated on simulations, with the effect of variation in velocity and the height of tracked features on reconstruction accuracy being investigated, with the results indicating this technique is suitable in real-world conditions. This framework is generalised, removing the need for prior knowledge of the number of planes. The problem is reformulated as a linear-series of planes, each connected by a single hinge, allowing the calculation of a single rotation for each new plane. Again, results are shown against simulations on scenes of varying complexity, as well as realworld datasets validating the success of this method given realistic variations in velocity

    Innovative 3D and 2D machine vision methods for analysis of plants and crops in the field

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    Ā© 2018 Elsevier B.V. Machine vision systems offer great potential for automating crop control, harvesting, fruit picking, and a range of other agricultural tasks. However, most of the reported research on machine vision in agriculture involves a 2D approach, where the utility of the resulting data is often limited by effects such as parallax, perspective, occlusion and changes in background light ā€“ particularly when operating in the field. The 3D approach to plant and crop analysis described in this paper offers potential to obviate many of these difficulties by utilising the richer information that 3D data can generate. The methodologies presented, such as four-light photometric stereo, also provide advanced functionalities, such as an ability to robustly recover 3D surface texture from plants at very high resolution. This offers potential for enabling, for example, reliable detection of the meristem (the part of the plant where growth can take place), to within a few mm, for directed weeding (with all the associated cost and ecological benefits) as well as offering new capabilities for plant phenotyping. The considerable challenges associated with robust and reliable utilisation of machine vision in the field are also considered and practical solutions are described. Two projects are used to illustrate the proposed approaches: a four-light photometric stereo apparatus able to recover plant textures at high-resolution (even in direct sunlight), and a 3D system able to measure potato sizes in-the-field to an accuracy of within 10%, for extended periods and in a range of environmental conditions. The potential benefits of the proposed 3D methods are discussed, both in terms of the advanced capabilities attainable and the widespread potential uptake facilitated by their low cost

    A Deep 150 MHz GMRT Survey in Eridanus

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    We present results of a 150 MHz survey of a field centered on Epsilon Eridani, undertaken with the Giant Metrewave Radio Telescope (GMRT). The survey covers an area with a diameter of 2 deg, has a spatial resolution of 30" and a noise level of 3.1 mJy at the pointing centre. These observations provide a deeper and higher resolution view of the 150 MHz radio sky than the 7C survey (although the 7C survey covers a much larger area). A total of 113 sources were detected, most are point-like, but 20 are extended. We present an analysis of these sources, in conjunction with the NVSS (at 1.4 GHz) and VLSS (at 74 MHz). This process allowed us to identify 5 Ultra Steep Spectrum (USS) radio sources that are candidate high redshift radio galaxies (HzRGs). In addition, we have derived the dN/dS distribution for these observations and compare our results with other low frequency radio surveys.Comment: 12 pages, 11 figures, 3 tables, accepted for publication in MNRA

    Generation of Ļ0 cells utilizing a mitochondrially targeted restriction endonuclease and comparative analyses

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    Eukaryotic cells devoid of mitochondrial DNA (Ļ0 cells) were originally generated under artificial growth conditions utilizing ethidium bromide. The chemical is known to intercalate preferentially with the mitochondrial double-stranded DNA thereby interfering with enzymes of the replication machinery. Ļ0 cell lines are highly valuable tools to study human mitochondrial disorders because they can be utilized in cytoplasmic transfer experiments. However, mutagenic effects of ethidium bromide onto the nuclear DNA cannot be excluded. To foreclose this mutagenic character during the development of Ļ0 cell lines, we developed an extremely mild, reliable and timesaving method to generate Ļ0 cell lines within 3ā€“5 days based on an enzymatic approach. Utilizing the genes for the restriction endonuclease EcoRI and the fluorescent protein EGFP that were fused to a mitochondrial targeting sequence, we developed a CMV-driven expression vector that allowed the temporal expression of the resulting fusion enzyme in eukaryotic cells. Applied on the human cell line 143B.TKāˆ’ the active protein localized to mitochondria and induced the complete destruction of endogenous mtDNA. Mouse and rat Ļ0 cell lines were also successfully created with this approach. Furthermore, the newly established 143B.TKāˆ’ Ļ0 cell line was characterized in great detail thereby releasing interesting insights into the morphology and ultra structure of human Ļ0 mitochondria

    SuperCLASS - II. Photometric redshifts and characteristics of spatially resolved mu Jy radio sources

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    We present optical and near-infrared imaging covering a similar to 1.53 deg(2) region in the Super-Cluster Assisted Shear Survey (SuperCLASS) field, which aims to make the first robust weak lensing measurement at radio wavelengths. We derive photometric redshifts for approximate to 176 000 sources down to i'(AB) similar to 24 and present photometric redshifts for 1.4 GHz expanded Multi-Element Radio Linked Interferometer Network (e-MERLIN) and Karl G. Jansky Very Large Array (VLA) detected radio sources found in the central 0.26 deg(2). We compile an initial catalogue of 149 radio sources brighter than S-1.4 > 75 mu Jy and find their photometric redshifts span 0 = 0.55 and = 1.9 x 10(23) WHz(-1), respectively. We find 95 per cent of the mu Jy radio source sample (141/149) have spectral energy distributions (SEDs) best fit by star-forming templates while 5 per cent (8/149) are better fit by active galactic nuclei (AGN). Spectral indices are calculated for sources with radio observations from the VLA and Giant Metrewave Radio Telescope (GMRT) at 325 MHz, with an average spectral slope of alpha = 0.59 +/- 0.04. Using the full photometric redshift catalogue, we construct a density map at the redshift of the known galaxy clusters, z = 0.20 +/- 0.08. Four of the five clusters are prominently detected at > 7 sigma in the density map and we confirm the photometric redshifts are consistent with previously measured spectra from a few galaxies at the cluster centres

    Comparison of diffusion tensor imaging by cardiovascular magnetic resonance and gadolinium enhanced 3D image intensity approaches to investigation of structural anisotropy in explanted rat hearts

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    Background: Cardiovascular magnetic resonance (CMR) can through the two methods 3D FLASH and diffusion tensor imaging (DTI) give complementary information on the local orientations of cardiomyocytes and their laminar arrays. Methods: Eight explanted rat hearts were perfused with Gd-DTPA contrast agent and fixative and imaged in a 9.4T magnet by two types of acquisition: 3D fast low angle shot (FLASH) imaging, voxels 50ā€‰Ć—ā€‰50ā€‰Ć—ā€‰50 Ī¼m, and 3D spin echo DTI with monopolar diffusion gradients of 3.6 ms duration at 11.5 ms separation, voxels 200ā€‰Ć—ā€‰200ā€‰Ć—ā€‰200 Ī¼m. The sensitivity of each approach to imaging parameters was explored. Results:The FLASH data showed laminar alignments of voxels with high signal, in keeping with the presumed predominance of contrast in the interstices between sheetlets. It was analysed, using structure-tensor (ST) analysis, to determine the most (v 1 ST ), intermediate (v 2 ST ) and least (v 3 ST ) extended orthogonal directions of signal continuity. The DTI data was analysed to determine the most (e 1 DTI ), intermediate (e 2 DTI ) and least (e 3 DTI ) orthogonal eigenvectors of extent of diffusion. The correspondence between the FLASH and DTI methods was measured and appraised. The most extended direction of FLASH signal (v 1 ST ) agreed well with that of diffusion (e 1 DTI ) throughout the left ventricle (representative discrepancy in the septum of 13.3ā€‰Ā±ā€‰6.7Ā°: medianā€‰Ā±ā€‰absolute deviation) and both were in keeping with the expected local orientations of the long-axis of cardiomyocytes. However, the orientation of the least directions of FLASH signal continuity (v 3 ST ) and diffusion (e 3 ST ) showed greater discrepancies of up to 27.9ā€‰Ā±ā€‰17.4Ā°. Both FLASH (v 3 ST ) and DTI (e 3 DTI ) where compared to directly measured laminar arrays in the FLASH images. For FLASH the discrepancy between the structure-tensor calculated v 3 ST and the directly measured FLASH laminar array normal was of 9ā€‰Ā±ā€‰7Ā° for the lateral wall and 7ā€‰Ā±ā€‰9Ā° for the septum (medianā€‰Ā±ā€‰inter quartile range), and for DTI the discrepancy between the calculated v 3 DTI and the directly measured FLASH laminar array normal was 22ā€‰Ā±ā€‰14Ā° and 61ā€‰Ā±ā€‰53.4Ā°. DTI was relatively insensitive to the number of diffusion directions and to time up to 72 hours post fixation, but was moderately affected by b-value (which was scaled by modifying diffusion gradient pulse strength with fixed gradient pulse separation). Optimal DTI parameters were bā€‰=ā€‰1000 mm/s2 and 12 diffusion directions. FLASH acquisitions were relatively insensitive to the image processing parameters explored. Conclusions: We show that ST analysis of FLASH is a useful and accurate tool in the measurement of cardiac microstructure. While both FLASH and the DTI approaches appear promising for mapping of the alignments of myocytes throughout myocardium, marked discrepancies between the cross myocyte anisotropies deduced from each method call for consideration of their respective limitations

    Guidelines for Modeling and Reporting Health Effects of Climate Change Mitigation Actions.

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    BACKGROUND: Modeling suggests that climate change mitigation actions can have substantial human health benefits that accrue quickly and locally. Documenting the benefits can help drive more ambitious and health-protective climate change mitigation actions; however, documenting the adverse health effects can help to avoid them. Estimating the health effects of mitigation (HEM) actions can help policy makers prioritize investments based not only on mitigation potential but also on expected health benefits. To date, however, the wide range of incompatible approaches taken to developing and reporting HEM estimates has limited their comparability and usefulness to policymakers. OBJECTIVE: The objective of this effort was to generate guidance for modeling studies on scoping, estimating, and reporting population health effects from climate change mitigation actions. METHODS: An expert panel of HEM researchers was recruited to participate in developing guidance for conducting HEM studies. The primary literature and a synthesis of HEM studies were provided to the panel. Panel members then participated in a modified Delphi exercise to identify areas of consensus regarding HEM estimation. Finally, the panel met to review and discuss consensus findings, resolve remaining differences, and generate guidance regarding conducting HEM studies. RESULTS: The panel generated a checklist of recommendations regarding stakeholder engagement: HEM modeling, including model structure, scope and scale, demographics, time horizons, counterfactuals, health response functions, and metrics; parameterization and reporting; approaches to uncertainty and sensitivity analysis; accounting for policy uptake; and discounting. DISCUSSION: This checklist provides guidance for conducting and reporting HEM estimates to make them more comparable and useful for policymakers. Harmonization of HEM estimates has the potential to lead to advances in and improved synthesis of policy-relevant research that can inform evidence-based decision making and practice. https://doi.org/10.1289/EHP6745

    SuperCLASS - III. Weak lensing from radio and optical observations in Data Release 1

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    We describe the first results on weak gravitational lensing from the SuperCLASS survey: the first survey specifically designed to measure the weak lensing effect in radio-wavelength data, both alone and in cross-correlation with optical data. We analyse 1.53 deg(2) of optical data from the Subaru telescope and 0.26 deg(2) of radio data from the e-MERLIN and VLA telescopes (the DR1 data set). Using standard methodologies on the optical data only we make a significant (10 sigma) detection of the weak lensing signal (a shear power spectrum) due to the massive supercluster of galaxies in the targeted region. For the radio data we develop a new method to measure the shapes of galaxies from the interferometric data, and we construct a simulation pipeline to validate this method. We then apply this analysis to our radio observations, treating the e-MERLIN and VLA data independently. We achieve source densities of 0.5 arcmin(-2) in the VLA data and 0.06 arcmin(-2) in the e-MERLIN data, numbers which prove too small to allow a detection of a weak lensing signal in either the radio data alone or in cross-correlation with the optical data. Finally, we show preliminary results from a visibility-plane combination of the data from e-MERLIN and VLA which will be used for the forthcoming full SuperCLASS data release. This approach to data combination is expected to enhance both the number density of weak lensing sources available, and the fidelity with which their shapes can be measured
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